Figure 2.

RT-PCR detects SPIK RNA. 1μg RNA from HepG2 cells and human liver cells were reversely transcripted and amplified by PCR using specific primer for SPIK. PCR bands were resolved in 1 % agarose gel and stain with ethidium Bromide. A: The names and location of primers. B: RT-PCR results. Lanes 1 to 4 were amplified with primers PI1/PI2; 5 to 8 were amplified with primers PI3/PI4. The samples from human liver cells were loaded in the column 1,2,5,6, (duplicated). The samples from HepG2 were loaded in the lane 3,4,7,8. Lane 9 was negative control without RTase. The specific SPIK bands are indicated by molecular weight. The lower bands in B are dimmer of primers. C: RT-PCR detection of TH gene of the human liver cells and HepG2 cells.