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Southern blot detection of HBV DNA in the infected HepG2 cells. 106 HepG2 cells were pre-treated with anti-sense or sense oligo of SPIK or left untreated, then infected by patients' serum. Cells were harvested at 8th day p.i. After removal of nuclei, HBV DNA in cytoplasma was isolated, resolved in 1% agarose gel and detected by southern blot with HBV specific probe.
