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. 2023 Nov 17;10(12):uhad230. doi: 10.1093/hr/uhad230

Figure 2.

Figure 2

Sequence analysis and functional verification of LbCCD4.1. A Multiple sequence alignment of the CCD4.1 genes in NQ, NX and HG. B Subcellular localization of LbCCD4.1 in tobacco leaves. Scale bars = 50 μm. C The qRT-PCR detection of LbCCD4.1 expression in the leaves of NQ OE-LbCCD4.1 plants using GFP as the control. D Detection of carotenoid metabolites in the leaves of NQ LbCCD4.1 over-expression plants. Three biological replicates were used, and values are presented as averages ± standard deviations. Student’s t-test was used to determine significance: *P < 0.05; **P < 0.01; ***P < 0.001.