FIG. 1.

Circular dichroism analysis of purified OmpG. Purification and measurement were carried out as described in Materials and Methods. The magnitude of mean residue ellipticity (about −5,000 deg² dmol⁻¹) is significantly smaller than that found with OmpA or P. aeruginosa OprF (−9,000 to −10,000 deg² dmol⁻¹ [38]), two outer membrane proteins that contain substantial α-helical domains. The spectrum also does not show the two sharp negative peaks at 208 and 220 nm, which are characteristic of α helices.