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Immunoblot analysis of OmpG from envelopes of various gram-negative bacteria. Lanes 1 and 2 contain envelopes (1.5 μg of protein) from OmpG− and OmpG+ E. coli strains, respectively; lanes 3 to 7 contain 0.75, 1.5, 3, 6, and 12 μg of total protein, respectively. Gels were blotted with polyclonal antibodies raised against E. coli OmpG.
