Fig 2.

 Two-dimensional (2D) electrophoretic separation of total proteins isolated from adult flies (A) Control 25°C; (B) heat shock 38.5°C 30 minute plus 3 hours of recovery at 25°C. Silver stain. Inducible heat shock protein 70 members are indicated by arrows. Gel resulted from 2D separation of proteins (Drosophila virilis strain 1433) stained with Coomassie G-250 used for the isolation of 2 electromorphs of interest. (C) Gel before isolation. (D) The same gel with the excised spots indicated by arrows