Fig. 4. Contributions of PRC2 stimulation by H3K27me3.
(A) The simulated H3K37me3 profiles over the H15 locus during five cell cycles after removing PREs. Shown are the differences in the H3K27me3 occurrence relative to a chromatin fiber without PREs. The occurrence was calculated from five inspections within one cell cycle over 1000 parallel simulations. (B) Box plots showing the percentage of H3K27 positions in each methylation state from 10 random inspections of 1000 different simulations (when they have reached the steady state) performed with (orange; S3 = 3.5 and kb = 0.625 × 10−4) or without (green; S3 = 1 and kb = 1.125 × 10−4) H3K27me3 stimulation. The means are shown as yellow dashed lines. The average percentages of corresponding methylated H3K27 molecules measured by mass spectrometry (33) are shown for comparison. (C) The mean H3K37me3 profiles over the trachealess (trh) locus simulated with (orange line) and without (green line) H3K27me3 stimulation captured during the first, the middle, and the last inspections of the cell cycle. The 95% confidence intervals from 1000 simulations are shown as shaded areas.