Table 1.

Common changes observed in A. vinelandii MoFe-protein structures. Cells highlighted in red indicate a deviation from the resting state (MoFe^As-isolated) structure (PDB code 3U7Q). Flip indicates an alternate conformation is observed. N/C stands for no change.

	MoFeAs-isolated PDB: 3U7Q	MoFe∆nifB PDB: 1L5H	MoFe∆nifV PDB: 8ENN		MoFeAlkaline-inactivated PDB: 8ENL		t/oComplex 1 PDB: 7UT8		t/oComplex 2 PDB: 7UT9		MoFeAcidic PDB: 5VQ4	MoFeCO PDB: 4TKV	MoFeCO-CO PDB: 7JRF	MoFeSe PDB: 5BVG
Cα RMSD with 3U7Q		0.370	0.385		0.387		0.405		0.433		0.257	0.101	0.090	0.077
Asymmetric	No	No	Yes		Yes		Yes		Yes		No	No	No	No
Disordered	No	Yes	Yes		Yes		Yes		Yes		No	No	No	No
Ordered/Disordered dimer			Ord.	Dis.	Ord.	Dis.	Ord.	Dis.	Ord.	Dis.
α-Gln191	Reference	Flip	N/C	Flip	N/C	Flip	N/C	N/C	N/C	N/C	N/C	N/C	N/C	N/C
α-Trp253	Reference	Flip	N/C	Flip	N/C	Flip	N/C	Flip	N/C	Flip	Flip	N/C	N/C	N/C
α-His274	Reference	Flip	N/C	Flip	Flip	Flip	N/C	Flip	N/C	Flip	Flip	N/C	N/C	N/C
α-Phe300	Reference	Flip	N/C	Flip	Flip	Flip	N/C	Flip	N/C	Flip	Flip	N/C	N/C	N/C
α-His362	Reference	Flip	N/C	N/C	N/C	Flip	N/C	N/C	N/C	N/C	Flip	N/C	N/C	N/C
α-His451	Reference	Flip	N/C	Flip	Flip	Flip	N/C	Flip	N/C	Flip	N/C	N/C	N/C	N/C
β-Gln93	Reference	Flip	N/C	N/C	N/C	Flip	N/C	N/C	N/C	N/C	N/C	N/C	N/C	N/C