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. 2023 Dec 14;12(12):1452. doi: 10.3390/pathogens12121452

Figure 1.

Figure 1

Viral DNA in oral swabs and viral transcripts detected in the infected tongues. We followed viral replication in the infected mice by collecting longitudinal oral swabs and analyzed viral DNA by qPCR. Significantly higher levels of viral DNA were detected in the oral swabs of Rag1ko mice when compared to the infected B6 mice ((A), * p < 0.05, Mann–Whitney rank sum test) at different time points post-viral infection. No viral DNA was detected in B6 mice at week six post-viral infection. The infected tongues were harvested for viral RNA analysis by qRT-PCR at week four post-viral infection. Viral RNA transcripts were detected in some infected tongues of different mouse strains (Table 2). While more than half of B6 and Rag1ko mice were positive for viral transcripts, two CCR2ko and four Stat1ko mice (out of six each) had very low levels of viral RNA at week four post-viral infection. No significant difference was found between the different groups of animals as well as different sexes ((B), p > 0.05, the Kruskal–Wallis test).