Effect of chloramphenicol on transcription. RNA was extracted from cells infected with phage c2 in the presence of chloramphenicol at 30 (B) or 50 (A, C, and D) μg/ml and analyzed by Northern hybridization to the following probes: A, EP1; B, EP2b; C, EP6; and D, LP1b. Four micrograms of total RNA per lane was used. Timepoints, transcript sizes, and rRNA amounts are as indicated for Fig. 2.