Table 1.
A summary of the bacterial growth parameters of Vp M0904, Vp TW01 and Vp PV1 with starting populations of 101 and 103 CFU/mL, derived from the modified Gompertz model. YM represents the maximum population, expressed as values of optical density (OD550); Lag represents the length of the lag time in h. Between brackets, significant differences between transferrin treatment and relative control (0 mg/mL) are indicated by asterisks: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Non-significant differences are indicated by ‘ns’. (/: modified Gompertz model was not able to calculate growth parameters due to too long lag phase or completely flat curve.)
| Vp M0904 | |||||
|---|---|---|---|---|---|
| Treatment (mg/mL) | bLF | OT | |||
| YM | Lag | YM | Lag | ||
| 101 CFU/mL | 10 | 0.075 (****) | / | 0.656 (****) | 13.375 (****) |
| 5 | 0.080 (****) | / | 0.598 (****) | 13.500 (****) | |
| 1 | 0.370 (****) | 16.382 (****) | 0.580 (****) | 11.757 (****) | |
| 0.1 | 0.588 (ns) | 10.700 (ns) | 0.601 (****) | 9.125 (ns) | |
| 0.01 | 0.458 (****) | 10.546 (*) | 0.485 (ns) | 9.509 (ns) | |
| 0.001 | 0.541 (****) | 11.084 (ns) | 0.457 (ns) | 9.527 (ns) | |
| 0 | 0.605 | 11.142 | 0.450 | 9.625 | |
| 103 CFU/mL | 10 | / | / | 0.675 (****) | 10.176 (****) |
| 5 | / | / | 0.626 (****) | 10.068 (****) | |
| 1 | 0.493 (****) | 11.972 (****) | 0.598 (****) | 8.160 (**) | |
| 0.1 | 0.653 (*) | 8.644 (ns) | 0.571 (****) | 7.481 (ns) | |
| 0.01 | 0.610 (ns) | 7.907 (ns) | 0.498 (***) | 7.478 (ns) | |
| 0.001 | 0.647 (*) | 8.396 (ns) | 0.474 (ns) | 7.213 (ns) | |
| 0 | 0.613 | 8.475 | 0.451 | 7.098 | |
| Vp TW01 | |||||
| Treatment (mg/mL) | bLF | OT | |||
| YM | Lag | YM | Lag | ||
| 101 CFU/mL | 10 | / | / | 0.801 (*) | 13.694 (****) |
| 5 | / | / | 0.908 (***) | 12.802 (****) | |
| 1 | 0.530 (ns) | 18.256 (****) | 1.008 (****) | 9.078 (ns) | |
| 0.1 | 0.576 (ns) | 8.664 (*) | 1.036 (****) | 7.392 (ns) | |
| 0.01 | 0.572 (ns) | 8.977 (ns) | 0.801 (ns) | 8,450 (ns) | |
| 0.001 | 0.581 (ns) | 9.588 (*) | 0.751 (ns) | 8.248 (ns) | |
| 0 | 0.558 | 9.148 | 0.695 | 8.051 | |
| 103 CFU/mL | 10 | / | / | 0.977 (****) | 9.181 (****) |
| 5 | / | / | 1.027 (****) | 8.723 (***) | |
| 1 | 0.498 (****) | 12.184 (****) | 1.070 (****) | 6.106 (ns) | |
| 0.1 | 0.564 (ns) | 6.439 (ns) | 1.088 (****) | 5.123 (ns) | |
| 0.01 | 0.560 (ns) | 6.532 (ns) | 0.804 (ns) | 5.394 (ns) | |
| 0.001 | 0.574 (ns) | 6.643 (ns) | 0.731 (ns) | 5.711 (ns) | |
| 0 | 0.561 | 6.624 | 0.724 | 5.825 | |
| Vp PV1 | |||||
| Treatment (mg/mL) | bLF | OT | |||
| YM | Lag | YM | Lag | ||
| 101 CFU/mL | 10 | / | / | 0.561 (****) | 16.184 (****) |
| 5 | / | / | 0.661 (****) | 13.651 (****) | |
| 1 | 0.523 (****) | 13.489 (****) | 1.290 (**) | 12.527 (****) | |
| 0.1 | 1.137 (ns) | 8.645 (ns) | 1.559 (ns) | 8.977 (ns) | |
| 0.01 | 1.447 (***) | 7.303 (ns) | 1.564 (ns) | 9.411 (ns) | |
| 0.001 | 1.065 (ns) | 8.107 (ns) | 1.586 (ns) | 9.058 (ns) | |
| 0 | 1.029 | 8.191 | 1.668 | 8.613 | |
| 103 CFU/mL | 10 | / | / | 0.621 (****) | 12.428 (****) |
| 5 | / | / | 0.964 (****) | 11.916 (****) | |
| 1 | 0.528 (***) | 9.032 (***) | 1.429 (ns) | 8.674 (***) | |
| 0.1 | 1.204 (ns) | 5.386 (ns) | 1.544 (ns) | 6.848 (ns) | |
| 0.01 | 1.615 (ns) | 5.110 (ns) | 1.554 (ns) | 6.760 (ns) | |
| 0.001 | 1.534 (ns) | 4.845 (ns) | 1.585 (ns) | 6.687 (ns) | |
| 0 | 1.375 | 4.584 | 1.565 | 6.939 | |