Table 2.
In vitro antiproliferative activity of nitrostyrene compounds 11g, 11h, 11i and 11j in HeLa, MCF-7, HL-60, Ramos, Bjab, MUTU-I and DG-75 cell lines a.
| % Viable Cells Remaining | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HeLa 24 h |
MCF-7 72 h |
Ramos 24 h |
Bjab 24 h |
HL60 24 h |
MUTU-1 b 24 h |
DG-75 b 48 h |
||||||||
| 10 µM |
1 µM |
10 µM |
1 µM |
10 µM |
1 µM |
10 µM |
1 µM |
10 µM |
1 µM |
10 µM |
1 µM |
10 µM |
1 µM |
|
| 11g | 34.2 | 87.1 | 41.7 | 81.4 | 7.7 | 53.4 | 51.9 | 82.2 | 16.5 | 73.4 | 14.91 | 44.16 | −0.08 | 75.86 |
| 11h | 23.2 | 90.6 | 29.7 | 94.3 | 5.1 | 27.2 | 46.2 | 90.1 | 6.8 | 78.2 | 10.28 | 39.80 | 0.38 | 84.50 |
| 11i | 17.7 | 93.0 | 37.1 | 102.7 | 13.9 | 62.6 | 50.8 | 100.5 | 6.5 | 103.8 | 10.53 | 67.22 | 0.72 | 88.76 |
| 11j | 21.8 | 95.3 | 20.7 | 98.1 | 4.4 | 66.9 | 68.9 | 102.4 | 9.1 | 99.8 | 10.27 | 64.31 | 15.89 | 79.77 |
| Taxol | 48.4 | 86.9 | 75.0 | 100.8 | 24.9 | 69.3 | 80.2 | 97.6 | 69.7 | 97.6 | 7.0 | 32.0 | 40.0 | >90 |
a Cell proliferation of HeLa, MCF-7, HL-60, Ramos, Bjab, MUTU-I and DG-75 cells was determined with an alamarBlue assay. Compound concentrations of either 1 μM or 10 μM for 24 h (HeLa, Ramos, Bjab, HL60 and MUTU-I) or 48 h (DG-75) or 72 h (MCF-7) were used to treat the cells (in triplicate) with control wells containing vehicle ethanol (1% v/v). The mean value for three independent experiments is shown b [39].