Fig 5.

ATG5-binding domain of ATG16L1 is required for IFNγ-induced restriction of norovirus replication in BV-2 cells. (A) Schematic representation of ATG16L1 complementation in clone 1 Atg16l1^−/− BV-2 cells. (B) Intracellular staining and detection of TY1 epitope in complemented cells by flow cytometry. (C) Viability assay of Atg16l1^VECTOR, Atg16l1^WT, Atg16l1^ΔT300A, Atg16l1^ΔD299E, Atg16l1^ΔWipi2, Atg16l1^ΔWD, Atg16l1^ΔFip200, Atg16l1^ΔAtg5, and Atg16l1^VECTOR complemented BV-2 cells as described in Fig. S1. Values in (C) represent means ± SEM from three independent experiments. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, and ****P ≤ 0.0001 were considered statistically significant. ns, not significant. P value was determined by two-way ANOVA with Dunnett’s multiple comparison test.