Figure 4.

(a) Cleavage of the mutant TP53 gene amplicon (1100 bp, 160 and ¹80 ng) with PEI-GQD/RNP (Cas9 (5 pmol) and sgRNA (12 pmol)) complex along with PEI-GQD and RNP alone in the adjacent the gel lanes. The rightmost lane represents intact DNA control. (b) In vitro mismatch cleavage assay of the TP53 gene amplicon (400 bp) performed with the addition of T7E1 restriction enzyme. PC-3 cells were transfected with PEI-GQD/RNP (in ¹reaction buffer, ²3X reaction buffer, and ³3X opti-mem), and Lipofectamine/RNP (⁴with 0.5 μl of 500 mM of EDTA).