Table 1:
Thermocycling profile for PCR amplification
| Step | Temperature (°C) | Time (sec) | Cycle |
|---|---|---|---|
| Initial Denaturation | 98.0 | 10 | 1× |
| Denaturation | 98.0 | 1 | 30× |
| Annealing | 63.5 | 5 | |
| Elongation | 72.0 | 10 | |
| Final Extension | 72.0 | 60 | 1× |
| Hold | 4.0 | ∞ | 1× |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.