Table 2:
Thermocycling profile for in vitro mismatch cleavage assay
| Thermocycling profile | ||
|---|---|---|
| Step | Temperature (°C) | Time (sec) |
| Initial Denaturation | 95 | 300 |
| Annealing | 95 – 85 | −2°C/s |
| 20 | −0.1°C/s | |
| Hold | 4 | ∞ |
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