Fig 4.

Depletion of PPKL does not affect the replication of centrosomes. Centrosome duplication of PPKL^AID grown with and without auxin was monitored by IFA using antibodies against centrin 1. (A) IFA of parasites grown with/without IAA for 18 hours and stained with anti-IMC3, anti-centrin 1 antibodies, and DapI. (B) Quantification (mean ± SD) of the number of centrosomes of single swollen PPKL^AID parasites treated with IAA for 18 hours. (C) IFA of PPKL^AID parasites grown with/without IAA for 6 hours. (D) Quantification (mean ± SD) of dividing PPKL^AID parasites treated with/without IAA for 6 hours with one or two daughters. Three biological replicates were performed, with 50 parasites counted for each replicate. ***P < 0.001 (Student’s t-test, two tails, unequal variance). (E) U-ExM of one daughter containing PPKL^AID parasites treated with IAA for 6 hours and stained with anti-acetylated tubulin, anti-centrin 1, NHS-ester, and Draq5. Scale bar: 5 µm.