Fig 5.
Depletion of PPKL reduces the stability of microtubules. The cortical cytoskeleton was extracted from PPKLAID parasites treated with IAA or ethanol for 6 hours. (A) IFA of the extracted cortical cytoskeleton used anti-acetylated tubulin and anti-IMC6 antibodies to monitor the microtubules and IMC, respectively. Examples of fragmented microtubules are indicated by white arrows. (B) Quantification of fragmented microtubules normalized to the number of cortical cytoskeletons. The bars indicate the average number of fragmented microtubules from each cortical cytoskeleton. The error bars represent standard deviations. Three biological replicates, each consisting of two experimental replicates, were performed. For each experimental replicate, 10 random fields of view were selected to count the number of cortical cytoskeleton and fragmented microtubules. **P < 0.01 (Student’s t-test, two tails, unequal variance).