TABLE 1.
Putative PPKL neighboring proteinsa
| Gene ID | Annotation | Fold change | P value | Peptides | Comments | |
|---|---|---|---|---|---|---|
| Control | PPKL-TurboID | |||||
| TGGT1_244380 | Cactin | INF | 1.09E-18 | 0 | 363 | c |
| TGGT1_293180 | NADP-specific glutamate dehydrogenase | INF | 3.58E-14 | 0 | 169 | |
| TGGT1_290170 | PPKL | INF | 4.18E-14 | 0 | 167 | |
| TGGT1_245560 | Hypothetical protein | INF | 1.54E-13 | 0 | 151 | |
| TGGT1_232340 | PPM2A | INF | 4.30E-12 | 0 | 116 | |
| TGGT1_313270 | Hypothetical protein | INF | 1.84E-11 | 0 | 103 | |
| TGGT1_269410 | Hypothetical protein | INF | 2.07E-11 | 0 | 102 | |
| TGGT1_311230 | BCC7 | 63.00 | 1.36E-10 | 2 | 126 | a |
| TGGT1_310220 | Hypothetical protein | INF | 1.56E-10 | 0 | 86 | |
| TGGT1_203780 | Hypothetical protein | INF | 1.56E-10 | 0 | 86 | |
| TGGT1_321620 | DRPB | INF | 2.06E-10 | 0 | 84 | b |
| TGGT1_243200 | IMC29 | INF | 3.60E-10 | 0 | 80 | a |
| TGGT1_291180 | Hypothetical protein | INF | 1.62E-09 | 0 | 70 | |
| TGGT1_227800 | EPS15 | INF | 2.23E-09 | 0 | 68 | b |
| TGGT1_244120 | Hypothetical protein | INF | 1.24E-08 | 0 | 58 | |
| TGGT1_220270 | IMC6 | INF | 1.48E-08 | 0 | 57 | a |
| TGGT1_214180 | EpsL | INF | 2.15E-08 | 0 | 55 | b |
| TGGT1_259640 | Nucleoporin autopeptidase | INF | 2.60E-08 | 0 | 54 | |
| TGGT1_294360 | Putative ubiquitin-specific protease 39 isoform 2 | INF | 4.65E-08 | 0 | 51 | c |
| TGGT1_268950 | Hypothetical protein | INF | 6.95E-08 | 0 | 49 | |
| TGGT1_275490 | Hypothetical protein | INF | 1.30E-07 | 0 | 46 | |
| TGGT1_311400 | SEC31A | INF | 1.99E-07 | 0 | 44 | b |
| TGGT1_212140 | Hypothetical protein | INF | 4.90E-07 | 0 | 40 | |
| TGGT1_253440 | SRPK1 | 50.00 | 5.70E-07 | 1 | 50 | c |
| TGGT1_292950 | AC10 | INF | 1.28E-06 | 0 | 36 | a |
| TGGT1_262150 | K13 | INF | 2.73E-06 | 0 | 33 | b |
| TGGT1_231640 | IMC1 | 8.54 | 2.94E-06 | 24 | 205 | a |
| TGGT1_231070 | Protein kinase | INF | 9.12E-06 | 0 | 29 | a |
| TGGT1_221660 | DEAD/DEAH box helicase domain-containing protein | INF | 9.12E-06 | 0 | 29 | c |
| TGGT1_204280 | DYRK1 | INF | 9.12E-06 | 0 | 29 | |
| TGGT1_207370 | Hypothetical protein | INF | 1.13E-05 | 0 | 28 | |
| TGGT1_313790 | Hypothetical protein | INF | 1.13E-05 | 0 | 28 | |
| TGGT1_230210 | IMC10 | INF | 1.46E-05 | 0 | 27 | a |
| TGGT1_201700 | SEC13 | 34.00 | 2.18E-05 | 1 | 34 | b |
| TGGT1_275350 | TBC domain-containing protein | INF | 3.23E-05 | 0 | 25 | b |
| TGGT1_265870A | Pantoate-beta-alanine ligase | INF | 3.23E-05 | 0 | 25 | |
| TGGT1_320080 | Hypothetical protein | INF | 3.87E-05 | 0 | 24 | |
| TGGT1_298610 | GYF domain-containing protein | INF | 3.87E-05 | 0 | 24 | |
| TGGT1_235340 | ISC1 | INF | 4.98E-05 | 0 | 23 | a |
| TGGT1_250700 | Hypothetical protein | INF | 9.51E-05 | 0 | 22 | |
| TGGT1_216000 | IMC3 | 13.00 | 1.02E-04 | 3 | 39 | a |
| TGGT1_228150 | Hypothetical protein | INF | 1.07E-04 | 0 | 21 | |
| TGGT1_214880 | AC4 | INF | 1.35E-04 | 0 | 20 | a |
| TGGT1_260580 | Hypothetical protein | INF | 1.35E-04 | 0 | 20 | |
| TGGT1_260600 | TgPuf1 | INF | 1.35E-04 | 0 | 20 | |
| TGGT1_223420 | DnaJ domain-containing protein | INF | 1.35E-04 | 0 | 20 | |
| TGGT1_285500 | Hypothetical protein | INF | 1.35E-04 | 0 | 20 | |
| TGGT1_237290 | Hypothetical protein | INF | 1.35E-04 | 0 | 20 | |
| TGGT1_263520 | SPM1 | INF | 1.35E-04 | 0 | 20 | a |
| TGGT1_231630 | IMC4 | 5.89 | 1.98E-04 | 18 | 106 | a |
| TGGT1_205380 | Fructose bisphosphatase I | INF | 2.60E-04 | 0 | 19 | |
| TGGT1_213392 | Surface antigen repeat-containing protein | INF | 2.60E-04 | 0 | 19 | |
| TGGT1_296010 | Phosphatidylinositol 3- and 4-kinase | INF | 2.60E-04 | 0 | 19 | |
| TGGT1_305340 | Corepressor complex CRC230 | INF | 2.94E-04 | 0 | 18 | |
| TGGT1_310440 | MORN1 | INF | 2.94E-04 | 0 | 18 | a |
| TGGT1_291950 | RNA recognition motif-containing protein | INF | 2.94E-04 | 0 | 18 | |
| TGGT1_294730 | Hypothetical protein | INF | 2.94E-04 | 0 | 18 | |
| TGGT1_230940 | Hypothetical protein | 6.45 | 3.25E-04 | 11 | 71 | |
| TGGT1_294610 | SET | INF | 6.62E-04 | 0 | 17 | |
| TGGT1_258540 | Phosphoglycerate mutase family protein | INF | 6.62E-04 | 0 | 17 | |
| TGGT1_313430 | Hypothetical protein | INF | 6.62E-04 | 0 | 17 | |
| TGGT1_219710 | Hypothetical protein | INF | 6.70E-04 | 0 | 16 | |
| TGGT1_280370 | Hypothetical protein | INF | 8.21E-04 | 0 | 15 | |
| TGGT1_244470 | RNG2 | INF | 8.21E-04 | 0 | 15 | a |
| TGGT1_260540 | IMC14 | INF | 1.53E-03 | 0 | 14 | a |
| TGGT1_273560 | Kinesin B | INF | 1.53E-03 | 0 | 14 | a |
| TGGT1_253430 | Putative asparagine synthetase | 6.50 | 1.69E-03 | 6 | 39 | |
| TGGT1_282070 | Hypothetical protein | INF | 1.82E-03 | 0 | 13 | |
| TGGT1_249440 | Hypothetical protein | INF | 1.82E-03 | 0 | 13 | |
| TGGT1_214970 | Putative DNA replication licensing factor | INF | 3.39E-03 | 0 | 12 | |
| TGGT1_286580 | IMC17 | INF | 3.39E-03 | 0 | 12 | a |
| TGGT1_310950 | AP2XI-3 | INF | 3.39E-03 | 0 | 12 | |
| TGGT1_306660 | RNA pseudouridine synthase superfamily protein | INF | 3.39E-03 | 0 | 12 | |
| TGGT1_270770 | PWI domain-containing protein | INF | 3.39E-03 | 0 | 12 | |
| TGGT1_218960 | AP2XII-1 | INF | 3.39E-03 | 0 | 12 | |
| TGGT1_248700 | IMC12 | 16.00 | 3.87E-03 | 1 | 16 | a |
| TGGT1_224850 | Putative polyadenylate-binding protein | 10.00 | 6.24E-03 | 2 | 20 | |
| TGGT1_201680 | Putative eukaryotic initiation factor-3 subunit 10 | 10.00 | 6.24E-03 | 2 | 20 | |
| TGGT1_212260 | Autoantigen p27 | INF | 7.36E-03 | 0 | 10 | |
| TGGT1_270890 | Hypothetical protein | INF | 7.36E-03 | 0 | 10 | |
| TGGT1_313910 | RNA recognition motif 2 protein | INF | 7.36E-03 | 0 | 10 | |
| TGGT1_204160 | GYF domain-containing protein | INF | 7.74E-03 | 0 | 11 | |
| TGGT1_313380 | ILP1 | 14.00 | 8.05E-03 | 1 | 14 | a |
| TGGT1_313010 | DDX6 | 5.75 | 0.012 | 4 | 23 | c |
| TGGT1_291140 | CCR4-Not complex component, Not1 protein | INF | 0.015 | 0 | 9 | |
| TGGT1_315700 | Hypothetical protein | INF | 0.015 | 0 | 9 | |
| TGGT1_313370 | Hypothetical protein | INF | 0.015 | 0 | 9 | |
| TGGT1_309140 | Transducin beta-like protein TBL1 | INF | 0.015 | 0 | 9 | |
| TGGT1_207060 | Ribonucleoside-diphosphate reductase small subunit | INF | 0.015 | 0 | 9 | |
| TGGT1_306220 | Hypothetical protein | INF | 0.015 | 0 | 9 | |
| TGGT1_262950 | Hypothetical protein | INF | 0.015 | 0 | 9 | |
| TGGT1_268630 | YagE family protein | INF | 0.016 | 0 | 8 | |
| TGGT1_276890 | Hypothetical protein | INF | 0.016 | 0 | 8 | |
| TGGT1_250820 | Hypothetical protein | 3.53 | 0.019 | 15 | 53 | |
| TGGT1_269290 | Hypothetical protein | INF | 0.031 | 0 | 7 | |
| TGGT1_258970 | Hypothetical protein | INF | 0.031 | 0 | 7 | |
| TGGT1_246950 | Hypothetical protein | INF | 0.031 | 0 | 7 | |
| TGGT1_273760 | HSP70 | 2.48 | 0.043 | 71 | 176 | |
a
Listed are proteins identified by TurboID that met the following criteria: (i) identified in both replicates and (ii) P value of Fisher’s exact test for the combined data is < 0.05. Proteins related to the IMC, apical complex, basal complex, and microtubules are commented as “a”; proteins related to vesicle transport are commented as “b”; and proteins related to RNA splicing are commented as “c.” The number of peptides listed is the total between the two replicates.