TABLE 2.
PPKL-dependent phosphopeptidesa
| Gene ID | Annotation | Peptides | Modification | Fold change (+IAA/−IAA) | ||
|---|---|---|---|---|---|---|
| 1 hour | 3 hours | 6 hours | ||||
| TGGT1_250830 | SAC3/GANP family protein | [R].ESSKDLHAEK.[T] | 1xPhospho [S] | 4.37 | 15.87 | 3.737 |
| TGGT1_263520 | Microtubule-associated protein SPM1 | [K].KLPSEEGSDYGYPQKPQK.[Y] | 2xPhospho [S4(100); S8(100)] | 2.12 | 12.83 | 3.099 |
| [K].KLPSEEGSDYGYPQKPQK.[Y] | 1xPhospho [S4(100)] | 2.43 | 14.78 | 5.256 | ||
| TGGT1_235470 | Myosin A | [R].SSDVHAVDHSGNVYK.[G] | 1xPhospho [S/Y] | 1.62 | 10.29 | 7.252 |
| TGGT1_321680 | Hypothetical protein | [K].EGREEEEEETASEEEDEHAEPK.[K] | 2xPhospho [T10(100); S12(100)] | 2.02 | 9.7 | 21.505 |
| TGGT1_278660 | Putative P-type ATPase4 | [R].RFSSKRESTVGGSGTGHSQLGK.[S] | 3xPhospho [S3(100); S4(100); S8(98.7)] | 1.57 | 5.89 | 4.058 |
| TGGT1_214270 | Putative translation initiation factor IF-2 | [K].DDSDDETKPAPPAK.[K] | 1xPhospho [S/T] | 1.98 | 2.53 | 2.772 |
| [R].RGGLSSDEEFEAK.[K] | 2xPhospho [S5(100); S6(100)] | 2.07 | 5.87 | 2.904 | ||
| TGGT1_265250 | RNA recognition motif-containing protein | [K].SKSPDFSELRK.[E] | 2xPhospho [S1(100); S3(100)] | 2.11 | 5.7 | 2.629 |
| TGGT1_209600 | Hypothetical protein | [K].EAQESSDDEDEDDAHFDGEDLK.[V] | 1xPhospho [S] | 1.87 | 4.51 | 2.718 |
| TGGT1_289650 | PEP carboxykinase I | [R].TQSSGSLKDSISFLEMLK.[K] | 2xPhospho [T/S] | 1.86 | 4.44 | 2.247 |
| TGGT1_269180 | MIF4G domain-containing protein | [R].RRGSNAGAAALPAGDGK.[V] | 1xPhospho [S4(100)] | 1.71 | 3.88 | 6.936 |
| TGGT1_248420 | Hypothetical protein | [R].SSSVSTIFK.[M] | 2xPhospho [S2(99.5); S3(100)] | 2.58 | 2.32 | 3.833 |
| TGGT1_291930 | RNA recognition motif-containing protein | [K].DVMMEEESDEDSDDEEKSERPVK.[K] | 2xPhospho [S12(100); S18(98.8)] | 0.49 | 0.25 | 0.257 |
| [K].NEKDVMMEEESDEDSDDEEK.[S] | 2xPhospho [S11(100); S15(100)] | 0.61 | 0.09 | 0.312 | ||
| TGGT1_288380 | Heat shock protein HSP90 | [K].SVDKEITESEDEEKPAEDAEEK.[K] | 2xPhospho [T7(100); S9(100)] | 0.58 | 0.1 | 0.282 |
| TGGT1_286790 | Nuclear factor NF2 | [K].KGLSADSDDASDKVSETKS.[-] | 2xPhospho [S11(100); S15(98)] | 0.42 | 0.16 | 0.193 |
| [K].KGLSADSDDASDKVSETK.[S] | 2xPhospho [S7(100); S11(99.2)] | 0.44 | 0.3 | 0.223 | ||
| [K].KGLSADSDDASDKVSETKS.[-] | 3xPhospho [S7(100); S11(100); S15(97.9)] | 0.53 | 0.15 | 0.136 | ||
| TGGT1_209060 | Thrombospondin type 1 domain-containing protein | [R].ENSQENQNAEPGETHAETEEVESNASEKLAK.[V] | 3xPhospho [S3(100); S23(98.5); S26(100)] | 0.61 | 0.15 | 0.21 |
| TGGT1_229490 | Tetratricopeptide repeat-containing protein | [R].NKWPGVEEESSDDGDKEGGGPSGMR.[K] | 2xPhospho [S10(100); S11(100)] | 0.59 | 0.15 | 0.239 |
| TGGT1_224850 | Putative polyadenylate-binding protein | [K].EGEDSGAEEEKEEEGQKR.[E] | 1xPhospho [S5(100)] | 0.58 | 0.18 | 0.24 |
| TGGT1_214270 | Putative translation initiation factor IF-2 | [K].DDSDDETKPAPPAK.[K] | 1xPhospho [S3(100)] | 0.66 | 0.18 | 0.313 |
| TGGT1_244650 | Putative eukaryotic initiation factor 5 | [K].KKADSDDDSDDDGQNGK.[E] | 2xPhospho [S5(100); S9(100)] | 0.39 | 0.2 | 0.206 |
| TGGT1_310170 | Hypothetical protein | [R].SGVTAPGGDKDTEELASDDDSDDEAGDKDEDGETNRVPGR.[D] | 2xPhospho [S17(100); S21(100)] | 0.64 | 0.21 | 0.289 |
| TGGT1_204160 | GYF domain-containing protein | [K].KKGDSDSEEDASEGDLATSR.[S] | 3xPhospho [S5(100); S7(100); S12(99.9)] | 0.58 | 0.22 | 0.295 |
| TGGT1_216410 | Hypothetical protein | [K].IVDDITADQENDESGKSDESDADKAESR.[E] | 3xPhospho [S14(100); S17(100); S20(100)] | 0.58 | 0.27 | 0.274 |
| TGGT1_264990 | Hypothetical protein | [R].QEGNDKVPQSAAPSASRQQSEENK.[R] | 2xPhospho [S] | 0.62 | 0.24 | 0.481 |
| TGGT1_321520 | Hypothetical protein | [K].CDWDKWIDSDDEDAK.[G] | 1xPhospho [S9(100)] | 0.61 | 0.26 | 0.222 |
| TGGT1_228400 | WD domain, G-beta repeat-containing protein | [K].NFPTPFEPKSDDDDDEDLDELR.[S] | 1xPhospho [S10(100)] | 0.55 | 0.28 | 0.442 |
| TGGT1_219150 | Zinc finger, zz-type domain-containing protein | [R].LLEMNVGCHEEKESDEDDGDKK.[R] | 1xPhospho [S14(100)] | 0.66 | 0.28 | 0.297 |
| TGGT1_297170 | Putative 50S ribosomal protein L17 | [R].RPSAAEWEESDSEEEKADSPSPYK.[M] | 2xPhospho [S10(98.5); S12(98.5)] | 0.61 | 0.34 | 0.265 |
| TGGT1_216730 | MCM2/3/5 family protein | [R].AAEDADEGEEIASQLQSLDLSDGSKKK.[R] | 2xPhospho [S21(99.1); S24(100)] | 0.51 | 0.36 | 0.391 |
| TGGT1_304970 | Cell cycle-associated protein kinase CDK, Crk1 | [R].LGSGNNFDEQKHQDSFR.[F] | 1xPhospho [S3(100)] | 0.55 | 0.4 | 0.379 |
| TGGT1_212770 | Hypothetical protein | [R].ERSDASRGPLVEALGGVDQTGADKDEK.[S] | 2xPhospho [S3(100); S6(100)] | 0.65 | 0.42 | 0.462 |
a
Listed are proteins with phosphopeptides whose numbers were either increased or decreased in PPKLAID parasites treated with auxin at all timepoints tested (1, 3, and 6 hours). For the 3- and 6-hour timepoint sample sets, phosphopeptides with FC >= 2 and FC <= 0.5 are listed. For the 1-hour timepoint sample set, phosphopeptides with FC >= 1.5 and FC <= 0.66 are listed. Phosphorylation sites in the peptides are underlined.