Fig 4.

ChIP-seq analysis of S. aureus WalR chromosome-binding sites. (A) Four response regulators with a C-terminal FLAG-tag for immunoprecipitation after induction with anhydrotetracycline in NRS384Δspa. (B) Dose-dependent production of WalR-FLAG (inset Western blot) with increasing concentrations of aTc. (C) Plot showing an increasing number of WalR ChIP-seq mapped reads (tag count) as the aTc concentration increases. (D) Summary of key ChIP-seq-binding sites across the S. aureus chromosome, showing binding motif and direction, including five WalR peaks (red), and single peaks for SaeR (green), VraR (blue), and HptR (yellow).