FIG 2.

Growth curves suggest that RecG may contribute to S. aureus ability to confer fitness under NO⋅ stress. A representative growth curve is shown for S. aureus WT JE2 and 15 DNA repair transposon mutants grown in tryptic soy broth with a mixture of 1 mM DEANO and 10 mM NOC-12 added at OD 0.2 (A) (n = 3). The amount of time it took each mutant strain to reach an OD of 0.6 with a mixture of 1 mM DEANO and 10 mM NOC-12 added at OD 0.2 (B) (n = 3). A representative growth curve is shown for S. aureus WT JE2 and 15 DNA repair transposon mutants grown in PNG with the addition of 10 mM DETA/NO at inoculum (C) (n = 3). Average terminal OD from growth curves (C) of S. aureus WT JE2 and 15 DNA repair transposon mutants grown in PNG minimal media with 10 mM DETA/NO added at inoculum (D) (n = 3). Data were analyzed via one-way analysis of variance with Dunnett’s multiple comparisons test for correction (*, P < 0.05) or Student’s two-tailed t-test (*, P < 0.05) where appropriate.