FIG 7.

Susceptibility of ays1Δ, mxr1Δ, srx1Δ, and trx1Δ mutants to HOCl and H₂O₂. (A) Transcriptional regulation of and description of the roles of AYS1, MXR1, SRX1, and TRX1 in reversing thiol oxidation. The log₂ fold change in gene expression after treatment with different oxidants was determined by RNAseq. The data are shown in Table S1, and the data are summarized in Fig. 4 and 5. Shaded boxes indicate significant P values. (B and D) Susceptibility of the ays1Δ, mxr1Δ, srx1Δ, and trx1Δ mutants to (B) HOCl and (D) H₂O₂. The x-axis reports the change in diameter of the zone of growth inhibition caused by spotting 5 µL of the indicated concentration of HOCl on a lawn of the indicated type of C. albicans cells. (C and E) Images of representative halo assays for the data shown in panels B and D. The results represent the average of three independent experiments, each done in duplicate. The trx1Δ strain grows poorly in minimal medium because Trx1 is also needed for methionine synthesis (37). Error bars indicate SD.