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Effects of mutating or overexpressing hrpV on the accumulation of Hrc proteins encoded by three different hrp operons. Bacteria grown for 5 h in Hrp minimal medium, as described in the legend to Fig. 1A, were assayed for the accumulation of HrcJ, HrcC, and HrcQB by SDS-polyacrylamide gel electrophoresis followed by immunoblot analysis with appropriate antibodies and chemiluminescent immunodetection. Pss61, P. syringae pv. syringae 61.
