Table 2.
Precision and accuracy data for withanoside IV, withanolide A and withaferin A.
| Analytes | Nominal concentration (ng/mL) | Intra-day (n = 6) |
Inter- day (n = 18) |
||||
|---|---|---|---|---|---|---|---|
| Observed concentration (ng/ml) |
Precision |
Accuracy |
Observed concentration (ng/ml) |
Precision |
Accuracy |
||
| Mean ± SD | (% CV) | Mean ± SD | Mean ± SD | (% CV) | Mean ± SD | ||
| Withanoside IV | LLOQ (1) | 0.88 ± 0.14 | 16.29 | 87.80 ± 16.29 | 0.96 ± 0.08 | 8.33 | 96.11 ± 8.29 |
| LQC (3) | 3.01 ± 0.34 | 11.41 | 100.12 ± 11.42 | 2.91 ± 0.09 | 3.00 | 96.86 ± 2.83 | |
| MQC (30) | 28.75 ± 3.41 | 11.87 | 95.84 ± 11.38 | 29.50 ± 0.80 | 2.71 | 98.33 ± 2.65 | |
| HQC (75) | 67.95 ± 3.85 | 6.94 | 90.60 ± 5.14 | 74.33 ± 5.91 | 7.95 | 99.01 ± 7.75 | |
| Withanolide A | LLOQ (1) | 0.95 ± 0.16 | 17.18 | 94.66 ± 16.23 | 0.90 ± 0.05 | 4.99 | 90.43 ± 4.08 |
| LQC (3) | 3.17 ± 0.10 | 3.24 | 105.73 ± 3.37 | 3.00 ± 0.16 | 5.24 | 100.03 ± 5.23 | |
| MQC (30) | 32.22 ± 1.15 | 3.57 | 107.43 ± 3.81 | 31.51 ± 1.00 | 3.18 | 105.03 ± 3.35 | |
| HQC (75) | 78.99 ± 2.78 | 3.52 | 105.32 ± 3.70 | 79.32 ± 0.38 | 0.48 | 104.76 ± 1.69 | |
| Withaferin A | LLOQ (0.5) | 0.50 ± 0.08 | 16.39 | 99.53 ± 16.25 | 0.52 ± 0.04 | 7.19 | 104.57 ± 7.24 |
| LQC (1.5) | 1.44 ± 0.12 | 8.07 | 95.73 ± 7.70 | 1.47 ± 0.07 | 5.03 | 97.74 ± 4.76 | |
| MQC (15) | 14.35 ± 1.00 | 6.98 | 95.65 ± 6.67 | 14.48 ± 0.20 | 1.36 | 96.55 ± 1.31 | |
| HQC (37.5) | 38.02 ± 2.93 | 7.71 | 101.38 ± 7.82 | 37.39 ± 2.51 | 6.72 | 98.33 ± 5.16 | |
LLOQ- Lower limit of quantification.
LQC- Lower quality control.
MQC-Middle quality control.
HQC- Higher quality control.