Table 3.
Extraction recovery and matrix effect of withanoside IV, withanolide A and withaferin A.
| Analytes | Nominal concentration (ng/mL) | R1 |
R2 |
R3 |
Recovery (%) |
Matrix effect (%) |
|---|---|---|---|---|---|---|
| Mean ± SD (n = 3) | Mean ± SD (n = 3) | Mean ± SD (n = 3) | Mean ± SD (n = 3) | Mean ± SD (n = 3) | ||
| Withanoside IV | LQC (3) | 14.67 ± 0.58 | 15.67 ± 0.58 | 16.67 ± 0.58 | 93.61 ± 0.24 | 94.00 ± 0.21 |
| MQC (30) | 170.00 ± 5.29 | 182.00 ± 3.00 | 200.00 ± 2.00 | 93.39 ± 1.52 | 91.00 ± 1.31 | |
| HQC (75) | 473.33 ± 5.03 | 508.67 ± 6.11 | 544.00 ± 7.21 | 93.06 ± 0.77 | 93.51 ± 1.14 | |
| Withanolide A | LQC (3) | 97.00 ± 1.00 | 106.33 ± 1.53 | 117.00 ± 1.00 | 91.23 ± 0.42 | 90.88 ± 0.56 |
| MQC (30) | 1308.33 ± 9.29 | 1433.67 ± 9.29 | 1534.67 ± 5.51 | 91.26 ± 1.02 | 93.42 ± 0.37 | |
| HQC (75) | 3371.33 ± 8.14 | 3680.00 ± 8.54 | 3993.33 ± 8.33 | 91.61 ± 0.27 | 92.15 ± 0.17 | |
| Withaferin A | LQC (1.5) | 275.33 ± 5.51 | 294.33 ± 4.04 | 313.00 ± 4.36 | 93.54 ± 0.66 | 94.04 ± 0.71 |
| MQC (15) | 1825.00 ± 2.00 | 1989.67 ± 7.37 | 2191.33 ± 5.86 | 91.72 ± 0.27 | 90.80 ± 0.55 | |
| HQC (37.5) | 4382.67 ± 5.86 | 4777.33 ± 8.62 | 5229.33 ± 6.81 | 91.74 ± 0.05 | 91.36 ± 0.13 |
R1 represents analytes spiked before extraction.
R2 represents analytes spiked after extraction.
R3 represents analytes prepared in injection solvent.
Recovery (%) was calculated as R1/R2 × 100.
Matrix effect (%) was calculated as R2/R3 × 100.