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. 2023 Dec 1;9(12):e23107. doi: 10.1016/j.heliyon.2023.e23107

Fig. 6.

Fig. 6

a) Gene expression analysis of meniscus matrix genes (ACAN, COL1A1, COL2A1) and SOX9 transcription factor for avascular and vascular meniscus cells cultured on 3D printed scaffolds (70 % infill). Data represent fold change in avascular and vascular meniscus cells seeded within the collagen scaffolds relative to corresponding monolayer meniscus cells for each donor (Data represent mean ± S.D.; n = 3). b) GAG and representative DMMB histological staining c) collagen I content of meniscus cells within 3D printed collagen scaffolds and representative collagen I histological staining (Data represent mean ± S.D.; n = 3; scale bar = 200 μm). d) collagen II histological staining for meniscus cells cultured on 3D printed scaffolds. (Scale bar: 200 μm). e) Biomechanical testing results, Values of E′ are shown separately and compared for samples seeded with cells and blank constructs as a function of the testing frequency. (Data represent mean ± S.D.; n = 6).