Table 1.

Composition of density gradient working buffer (pH 7.4)

Reagent	Final concentration	Amount
Sucrose	0.25 M	42.79 g
Tris-HCl	60 mM	4.725 g
EDTA (0.5 M)	6 mM	6 mL
Milli-Q ddH2O	N/A	qsf 500 mL
Total	N/A	500 mL