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. 2023 Nov 23;26(12):108567. doi: 10.1016/j.isci.2023.108567

Figure 4.

Figure 4

Confocal imaging of lipid environments targeted by ONOO− in live HeLa cells

(A–E) Cells were incubated with LNPs (50 μM) and stained with organelle trackers and/or actin dye (CellMask Deep Red Actin Tracking Stain) prior to imaging. LNPs obtained from a 47.5: 47.5:5.0 M ratio of DOTMA, DOPE, and DPPC-TC-ONOO–. (A) Cells treated with LNPs, stimulated with IFN-γ/LPS/PMA. Time coarse images acquired upon PMA treatment. (B) Cells treated with LNPs and 1400W. (C) Cells treated with LNPs, then with 1400W and IFN-γ/LPS/PMA. (D and E) Quantitative colocalization study of cells treated with LNPs and stimulated with IFN-γ/LPS/PMA. Organelle trackers: ER-Tracker, MitoTracker, CellLight Golgi-RFP, LysoTracker. DPPC-TC channel: 405/475 nm. Scale bars: (A–D) 20 μm, (E) 5 μm.