Figure 5.

Confocal imaging of lipid environments targeted by ONOO⁻ in live RAW246.7 cells

(A–E) Cells were seeded into glass microscope dishes, incubated with LNPs (50 μM) and stained with organelle trackers and/or actin dye prior to imaging. LNPs obtained from a 47.5: 47.5:5.0 M ratio of DOTMA, DOPE, and DPPC-TC-ONOO^–. (A) Cells treated with LNPs, stimulated with LPS. Time coarse images acquired after the addition of LPS. (B) Cells treated with LNPs and 1400W. (C) Cells treated with LNPs, then with 1400W and LPS. (D and E) Quantitative colocalization study of cells treated with LNPs and stimulated with LPS. Scale bars: (A–D) 20 μm or (E) 5 μm.