Antibodies |
|
FITC anti-mouse/human CD11b antibody |
BioLegend |
Cat # 101206; RRID: AB_312789
|
Alexa Fluor® 647 anti-mouse CD11c antibody |
BioLegend |
Cat # 117312; RRID: AB_389328
|
Alexa Fluor® 700 anti-mouse CD45 antibody |
BioLegend |
Cat # 103128; RRID: AB_493715
|
TruStainFcXanti-mouse CD16/32 |
BioLegend |
Cat # 101320; RRID: AB_1574975
|
|
Chemicals, peptides, and recombinant proteins |
|
Bleomycin |
Santa Cruz Biotechnology |
Cat # sc-200134B |
1-2-dipalmitoyl-rac-glycerol |
Millipore-Sigma |
CAS # 40290-32-2 |
1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) |
Avanti Polar Lipids |
CAS # 26853-31-6 |
1,2-palmitoyl-sn-glycero-3-phosphocholine (DPPC) |
Avanti Polar Lipids |
CAS # 63-89-8 |
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) |
Avanti Polar Lipids |
CAS # 384833-00-5 |
eFluor780-conjugated fixable viability dye |
Invitrogen |
Cat # 65-0865-14 |
ER-Tracker™ Green |
ThermoFischer Scientific |
Cat # E34251
|
MitoTracker Deep Red FM |
ThermoFischer Scientific |
Cat # M22426
|
CellLightTM Golgi-RFP, |
ThermoFischer Scientific |
Cat # C10593
|
LysoTrackerTM Deep Red |
ThermoFischer Scientific |
Cat # L12492
|
CellMask Deep Red Actin dye |
ThermoFischer Scientific |
Cat # A57248 |
Opti-MEM |
ThermoFischer Scientific |
Cat # 11058021 |
|
Critical commercial assays |
|
(MTT) cell proliferation assay kit |
ATCC |
30-1010K |
LDH kit |
Roche Applied Science |
06343767001 |
WST-1 reagent |
Roche Applied Science |
11644807001 |
|
Experimental models: Cell lines |
|
HeLa |
ATCC |
CRM-CCL-2 |
RAW 264.7 |
ATCC |
TIB-71 |
|
Experimental models: Organisms/strains |
|
C57BL6/J WT mice |
Jackson Laboratories |
Strain # 000664 |
NOS2-/- mice |
Jackson Laboratories |
Strain # 002609 |
|
Software and algorithms |
|
ChemDraw 22.2.0 |
PerkinElmer |
v22.2.0 |
GraphPad Prism |
GraphPad |
v8 |
ImageJ |
National Institutes of Health and the Laboratory for Optical and Computational Instrumentation |
https://imagej.nih.gov/ij/ |
Adobe Illustrator |
Adobe |
v2021 |
Imarius x64 |
Oxford Instruments |
v9.0.1 |
BioRender |
BioRender |
https://www.biorender.com/ |
Kaluza 2.1 |
Beckmann Coulter |
https://www.beckman.com/flow-cytometry/software/ |