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. 2023 Nov 23;26(12):108567. doi: 10.1016/j.isci.2023.108567
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

FITC anti-mouse/human CD11b antibody BioLegend Cat # 101206; RRID: AB_312789
Alexa Fluor® 647 anti-mouse CD11c antibody BioLegend Cat # 117312; RRID: AB_389328
Alexa Fluor® 700 anti-mouse CD45 antibody BioLegend Cat # 103128; RRID: AB_493715
TruStainFcXanti-mouse CD16/32 BioLegend Cat # 101320; RRID: AB_1574975

Chemicals, peptides, and recombinant proteins

Bleomycin Santa Cruz Biotechnology Cat # sc-200134B
1-2-dipalmitoyl-rac-glycerol Millipore-Sigma CAS # 40290-32-2
1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) Avanti Polar Lipids CAS # 26853-31-6
1,2-palmitoyl-sn-glycero-3-phosphocholine (DPPC) Avanti Polar Lipids CAS # 63-89-8
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) Avanti Polar Lipids CAS # 384833-00-5
eFluor780-conjugated fixable viability dye Invitrogen Cat # 65-0865-14
ER-Tracker™ Green ThermoFischer Scientific Cat # E34251
MitoTracker Deep Red FM ThermoFischer Scientific Cat # M22426
CellLightTM Golgi-RFP, ThermoFischer Scientific Cat # C10593
LysoTrackerTM Deep Red ThermoFischer Scientific Cat # L12492
CellMask Deep Red Actin dye ThermoFischer Scientific Cat # A57248
Opti-MEM ThermoFischer Scientific Cat # 11058021

Critical commercial assays

(MTT) cell proliferation assay kit ATCC 30-1010K
LDH kit Roche Applied Science 06343767001
WST-1 reagent Roche Applied Science 11644807001

Experimental models: Cell lines

HeLa ATCC CRM-CCL-2
RAW 264.7 ATCC TIB-71

Experimental models: Organisms/strains

C57BL6/J WT mice Jackson Laboratories Strain # 000664
NOS2-/- mice Jackson Laboratories Strain # 002609

Software and algorithms

ChemDraw 22.2.0 PerkinElmer v22.2.0
GraphPad Prism GraphPad v8
ImageJ National Institutes of Health and the Laboratory for Optical and Computational Instrumentation https://imagej.nih.gov/ij/
Adobe Illustrator Adobe v2021
Imarius x64 Oxford Instruments v9.0.1
BioRender BioRender https://www.biorender.com/
Kaluza 2.1 Beckmann Coulter https://www.beckman.com/flow-cytometry/software/