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. 2023 Nov 24;69:102972. doi: 10.1016/j.redox.2023.102972

Fig. 5.

Fig. 5

Oxidized SOD1 promotes MDV formation in early aging. (A) Reconstruction of MDVs from liver mitochondria. The yield of MDVs was determined by the protein content of MDVs (mg) per 380 mg of liver. (B) The ultrastructure of isolated MDVs from liver mitochondria by using TEM. Upper: lower magnification of MDV pellet. Scale bar, 500 nm. Lower: higher magnification of MDV with (a) double membranes, (b) a single membrane, (c) dense core, and (d) crescent shape. Scale bar, 100 nm. (C) Representative Western blot images of hSOD1 and misfolded SOD1 (A5C3) in young and middle-aged MDVs isolated from G93A and WT liver mitochondria. (D) Immunoblot analyses of biomarkers of MDVs (PDH and TOM20) from young and middle-aged groups. (E-H) Quantification of the protein levels of PDH and TOM20 in young and middle-aged MDVs (n = 3, mean ± SEM, **p < 0.01).