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. 2023 Nov 11;103(2):103209. doi: 10.1016/j.psj.2023.103209

Figure 4.

Figure 4

Circulating factors associated with mineral homeostasis. Circulating (A) ionized calcium and (B) bicarbonate were measured using CG8+ cartridges and an iSTAT hand-held blood analyzer at indicated hours post-oviposition (HPOP) (mean + SEM; n = 6 hens at 3, 4, 6, 7, 23, and 24 HPOP; n = 5 hens at 8, 12, and 21 HPOP; n = 4 hens at 1, 15, and 18 HPOP). (C) Plasma parathyroid hormone was measured using an ELISA (mean + SEM; n = 6 hens at 3, 4, 6, and 23 HPOP; n = 5 hens at 7, 8, 12, 21, and 24 HPOP; n = 4 hens at 1, 15, and 18 HPOP). (D) Plasma phosphorus was measured using Avian/Reptile Profile Plus cartridges and a VetScan2 (mean + SEM; n = 6 hens at 7, 23, and 24 HPOP; n = 5 hens at 12 HPOP; n = 4 hens at 1, 4, 8, 15, and 21 HPOP; n = 3 hens at 3,6, and 18 HPOP). Differences in available plasma volumes resulted in fewer hens analyzed at some time points. Data were analyzed by 1-way ANOVA followed by Fisher's test of least significant difference when ANOVA indicated significance, and bars not sharing a common letter differ significantly (P ≤ 0.05).