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. 2023 Nov 11;103(2):103209. doi: 10.1016/j.psj.2023.103209

Figure 6.

Figure 6

Expression of genes associated with calcium transport in kidney. Relative levels of mRNA were measured at indicated hours post-oviposition (HPOP) for kidney (A) CALB1, (B) NCX1, (C) PMCA1, and (D) TRPV6. Expression of target genes was determined by RT-qPCR and normalized to GAPDH mRNA. All values (mean + SEM; n = 6 hens at 3, 4, 6, 7, 23, and 24 HPOP; n = 5 hens at 8, 12, and 21 HPOP; n = 4 hens at 1, 15, and 18 HPOP) are expressed relative to 1 HPOP (equivalent to 1). Data were analyzed by 1-way ANOVA followed by Fisher's test of least significant difference when ANOVA indicated significance, and bars not sharing a common letter differ significantly (P ≤ 0.05).