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. 1998 Sep;180(17):4547–4554. doi: 10.1128/jb.180.17.4547-4554.1998

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Copyright © 1998, American Society for Microbiology

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FIG. 5 — Growth of wild-type and sigB mutant strains of L. monocytogenes after osmotic upshift in BHI (A) or DM (B and C). (A) LO4035 (wild type) and LMA2B (sigB::km) were grown in BHI, and the cultures were divided into two equal portions at 425 min (arrow). Sodium chloride was added to one portion to a final concentration of 6%, and both portions were further incubated. Symbols: ■, LO4035 without NaCl; □, LO4035 with 6% NaCl; •, LMA2B without NaCl; ○, LMA2B with 6% NaCl. (B) Equivalent volumes of 18-h cultures of LO4035 and LMA2B in DM were inoculated into DM without NaCl (▴, LO4035; ■, LMA2B), DM with 3% NaCl (✕⃒, LO4035; ×, LMA2B), and DM with 3% NaCl supplemented with 1 mM betaine (▵, LO4035; □, LMA2B. (C) Equivalent volumes of 18-h cultures of LO4035 and LMA2B in DM were inoculated into fresh DM (▴, LO4035; ■, LMA2B), DM with 3% NaCl (✕⃒, LO4035; ×, LMA2B), and DM with 3% NaCl supplemented with 1 mM carnitine (▵, LO4035; □, LMA2B).

FIG. 5 — Growth of wild-type and sigB mutant strains of L. monocytogenes after osmotic upshift in BHI (A) or DM (B and C). (A) LO4035 (wild type) and LMA2B (sigB::km) were grown in BHI, and the cultures were divided into two equal portions at 425 min (arrow). Sodium chloride was added to one portion to a final concentration of 6%, and both portions were further incubated. Symbols: ■, LO4035 without NaCl; □, LO4035 with 6% NaCl; •, LMA2B without NaCl; ○, LMA2B with 6% NaCl. (B) Equivalent volumes of 18-h cultures of LO4035 and LMA2B in DM were inoculated into DM without NaCl (▴, LO4035; ■, LMA2B), DM with 3% NaCl (✕⃒, LO4035; ×, LMA2B), and DM with 3% NaCl supplemented with 1 mM betaine (▵, LO4035; □, LMA2B. (C) Equivalent volumes of 18-h cultures of LO4035 and LMA2B in DM were inoculated into fresh DM (▴, LO4035; ■, LMA2B), DM with 3% NaCl (✕⃒, LO4035; ×, LMA2B), and DM with 3% NaCl supplemented with 1 mM carnitine (▵, LO4035; □, LMA2B).