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Recombinant plasmids used in this study. Numbers on the bars and lines denote the mass of each peptide or subunit, and the line stands for mature GL-7-ACA acylase gene. λPL, λPL promoter; Trx, thioredoxin-coding sequence; SP, signal peptide-coding sequence; T7, T7 promoter; Ser-199, Ser199 of a nascent GL-7-ACA acylase polypeptide or the N-terminal Ser residue of wild-type β subunit; 6XHis, sequence encoding six histidine residues. The plasmid pGAP18 has been described previously (14).
