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. 1998 Sep;180(17):4576–4582. doi: 10.1128/jb.180.17.4576-4582.1998

FIG. 3.

FIG. 3

SDS-PAGE analyses of the expression and processing patterns of TGA fusion proteins. Lane M, molecular mass markers; lanes T1 to T3, total cell extracts; lanes S1 to S3, soluble fractions; lanes P1 to P3, insoluble fractions from pTrxFus (vector)-, pTSG-, and pTNSG-harboring E. coli GI724, respectively. The upper and lower arrowheads in lane S3 denote the processed 54-kDa β subunit and 32-kDa TGα subunit, respectively. The arrow indicates each unprocessed 86-kDa protein aggregate.