FIG. 4.

Processing patterns of in vitro-expressed TGA fusion proteins in coupled in vitro transcription and translation reactions. Lane 1, in vitro-expressed β-galactosidase as a positive control; lanes 2 and 3, in vitro-expressed TGA fusion proteins from plasmids pACY and pSER, respectively. The upper and lower arrowheads in lanes 2 and 3 denote the processed β subunits and the TGα subunits, respectively, while the arrow denotes each unprocessed TGA fusion precursor. The mass of the TGα subunit in lane 2 is slightly higher than that of the TGα subunit in lane 3 because of the presence of the signal peptide in the fusion.