TABLE 1.
GL-7-ACA acylase activities of TGA fusion proteins expressed from pTSG- and pTNSG-harboring E. coli GI724 cells
Plasmid | Activity (U/ml of culture broth) in:
|
|
---|---|---|
Soluble fraction | Insoluble fraction | |
pTrxFus (vector) | NDa | ND |
pTSG | 0.29 | 0.24 |
pTNSG | 0.95 | 0.29 |
ND, not detectable.