TABLE 2.
GL-7-ACA acylase activities of reconstituted enzymes
| Component(s)a | Sp act (U/μg) | % Sp actb |
|---|---|---|
| WT TGα + WT Hβ | 0.012 | 19 |
| WT TGα + mutant Hβ | 0 | 0 |
| Mutant TGα + WT Hβ | 0.011 | 17.5 |
| Mutant TGα + mutant Hβ | 0 | 0 |
| Active TGAH protein | 0.063 | 100 |
a
WT, wild type. Mutant refers to subunits of Ser199Cys-mutated TGAH protein. Active TGAH protein was used for estimating the efficiency of enzyme reconstitution.
b
The percent specific activity of each reconstituted enzyme was calculated by comparison with the specific activity of active TGAH protein.