Table 3.
Molecular size of bands and polymorphism percentages according to the CRED-RA analysis.
| Primers | M*/H* 1 | ± 2 | Control 3 | Experimental Groups | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AgNO3 | Ag-NPs | |||||||||||
| 2 mg L−1 | 4 mg L−1 | 6 mg L−1 | 8 mg L−1 | 2 mg L−1 | 4 mg L−1 | 6 mgL−1 | 8 mg L−1 | |||||
| OPA 4 | M | + | 9 | - | 647; 585 | 576; 515; 402; 274 | 616; 564; 526 | 668; 600; 547 | 708; 622; 544; 508 | 654; 576; 505 | 691; 641; 564; 528; 512 | |
| - | - | 827 | - | 871; 827 | - | - | - | 827 | ||||
| H | + | 7 | 951; 330 | 907; 751; 600; 498; 433; 406; 289; 256; 227 | 1000; 916; 792; 550; 489; 433; 317; 294; 242 | 990; 541; 498; 472; 323; 160 | 1018; 907; 768; 641; 560; 489; 412; 298 | 907; 852; 792; 708; 553; 538; 519; 474; 416; 360; 330; 307 | - | 900; 871; 725; 491; 472; 319 | ||
| - | - | - | - | - | - | - | - | - | ||||
| OPH 18 | M | + | 15 | - | - | - | - | 1427; 800; 514 | 1427 | - | 1472 | |
| - | 1309; 337; 286 | 1145; 708; 582; 337; 286 | 1145; 1063; 882; 708; 666; 286 | 708; 337; 286 | 286 | 1145; 666; 286 | 708; 337; 286 | 420; 286 | ||||
| H | + | 11 | - | - | 471 | 1018; 832; 492; 359 | 467 | 380; 328 | 1145; 1000 | - | ||
| - | 1336; 1109; 775; 683; 627 | 1336; 891; 775; 683 | 1336; 1181; 891 | 1181 | 775 | 1109 | - | 627 | ||||
| OPW 4 | M | + | 11 | - | 731; 549 | - | - | 600; 434; 306 | 445 | 543; 310 | 800; 434 | |
| - | 362; 259; 205 | 362; 205 | 362; 205 | 205 | 305 | - | 841 | - | ||||
| H | + | 13 | 439 | - | - | 777; 434 | 296 | - | - | - | ||
| - | 900; 600; 327; 149 | 900; 629; 600; 516; 362; 149 | 900; 600 | 900; 629; 600; | 900; 629; 600; | 831; 600; 516 | 900; 600; 516 | 900; 831; 600; 570; 391 | ||||
| OPW 5 | M | + | 6 | 491 | 813;497; 338 | 1318; 864; 654 502; 338; 323 | 826; 494; 354;331; 305 | 852; 747; 578; 494; 406; 313 | 888; 760; 578; 482; 360; 338 | 864; 711; 639;570; 488; 403; 333; 297; 206 | 930; 839; 722; 632; 488 450; 346;320; 292 | |
| - | - | - | - | - | - | - | - | - | ||||
| H | + | 12 | 826 | 900; 760; 679; 600; 524; 510; 264 | 983; 930; 760; 604 | 826; 502 | 800; 711 | 921; 734; 600; 294 | 864 | 888; 773; 513; 292 | ||
| - | 639; 482; 394; 373; 352; 333 | - | 415; 394; 333; 252; 200 | 373 | 394; 373 | 394 | 532; 415; 333; 200 | 415; 394 | ||||
| OPW 6 | M | + | 11 | 829; 296 | 854; 749; 466; 145 | 800; 154 | 966; 866; 715 | 955; 866; 766 | 955; 866; 700; 641; 449; 352; 228;179 | 900; 800; 700; 312 | 900; 800; 715; 191 | |
| - | - | - | - | 485; 429 | - | - | 485 | - | ||||
| H | + | 12 | 605 | 922; 672 252; 150 | 955; 732 | 955; 749; 600; 472; 318; 296; 166 | 456; 432; 324; 179 | 866; 216 | 933; 863 | 429; 220; 154; 100 | ||
| - | 515 | 515 | 515 | 515 | 584; 539; 515 | - | - | 641; 539 | ||||
| OPW 11 | M | + | 9 | - | 646 | 670; 514 | - | - | - | 800; 145 | - | |
| - | - | 238 | 367; 293 | 722; 530; 238 | 722; 400; 293 | 428; 293 | - | - | ||||
| H | + | 6 | 300; 210 | 293 | - | 813; 575; 450; 378 | 455; 312; 232 | 504; 331; 268 | 437; 400 | 679; 495; 437 | ||
| - | - | - | 722; 419 | - | - | - | - | - | ||||
| OPW 17 | M | + | 5 | 788 | 324 | 547 | 180 | 860; 644; 563; 321 | 309 | 900; 810; 419 | 983; 886; 846 | |
| - | 488; 449 | 488 | 362 | - | 221 | 488; 449; 221 | - | 488; 268; 221 | ||||
| H | + | 3 | 860; 737; 609; 514; 465; 435; 375 | - | 724; 582 | 800; 502 | 913; 810; 700; 574; 547; 509 | 567; 524 | 880; 800; 631 | 838; 604; 377 | ||
| - | - | - | 321 | 265 | 321; 265 | 321; 265 | 321; 265 | 265 | ||||
| OPW 20 | M | + | 11 | - | 800; 713 | 710; 655; 512 | 900; 362 | 775; 524; 502; 384 | 818; 652; 561; 507; 306 | 655; 512; 287 | 818; 761; 649; 368; 324 | |
| - | 485; 469; 203 | 203 | 203 | 677; 203 | 203 | - | - | 540; 485 | ||||
| H | + | 9 | 717; 622 | 818; 619; 600 | 849; 734; 710; 605; 509 | 860; 717; 600; 514; 384 | 800; 734; 448; 386; 306; 219 | 818; 258 | 917; 791; 625;600; 386 | 880; 749; 710; 661; 512; 431; 362 | ||
| - | 258 | 258 | 258 | 258 | 634 | 258 | 258 | 258 | ||||
1 M—Msp I, H—Hpa II; 2 (+) appearance of a new band, and (-) disappearance of a normal band; 3 without AgNO3 and Ag-NPs, respectively.