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. 2023 Dec 14;15(12):2779. doi: 10.3390/pharmaceutics15122779

Figure 5.

Figure 5

EMSA of Ku-DNA complexes. 100 nM DNAs were incubated at 37 °C for 20 min with 15 μM HSA ((left) panel: lanes 2, 6; (right) panel: lanes 1, 5) or for 10 min with 15 μM HSA + 30/60 nM Ku ((left) panel: lanes 4, 8; (right) panel: lanes 3, 7), or 10 min with 15 μM HSA ((left) panel: lanes 3, 7; (right) panel: lanes 2, 6) followed by addition of 30/60 nM Ku with further incubation for 10 min, or 20 min with 30/60 nM Ku ((left) panel: lanes 5, 9; (right) panel: lanes 4, 8). Lanes 1 and 10 ((left) panel)—DNA, control without protein(s).