Figure 6.

Cross-linking of [³²P]-dRp-containing DNAs to Ku and HSA. 100 nM [³²P]-dRp-containing DNAs were incubated at 37 °C for 20 min with 15 μM HSA (lanes 5, 9) or for 10 min with 15 μM HSA + 60 nM Ku (lanes 3, 7), or for 10 min with 15 μM HSA (lanes 4, 8) followed by addition of 60 nM Ku with further incubation for 10 min or 20 min with 60 nM Ku ARP1 (lanes 2, 6). Lanes 1 and 10—DNA, control without protein(s). After incubation, the reaction mixtures were supplemented with 20 μM sodium borohydride to reduce the Schiff base for 30 min at 0 °C.