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. 2023 Dec 14;15(12):2779. doi: 10.3390/pharmaceutics15122779

Figure 8.

Figure 8

Cross-linking of [32P]-dRp-containing DNAs to PARP1 and HSA. 100 nM [32P]-dRp-containing DNAs were incubated at 37 °C for 20 min with 15 μM HSA (lanes 5, 9) or 10 min with 15 μM HSA + 0.15 μM PARP1 (lanes 3, 7), or 10 min with 15 μM HSA (lanes 4, 8) followed by addition of 0.15 nM PARP1 with further incubation for 10 min or 20 min with 0.15 μM PARP1 (lanes 2, 6). Lanes 1 and 10—DNA, control without protein(s). After incubation, the reaction mixtures were supplemented with 20 mM sodium borohydride to reduce the Schiff base for 30 min at 0 °C. The products were analyzed in 10% SDS-PAGE according to [97].