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. 2005 Apr 6;33(6):1993–2002. doi: 10.1093/nar/gki347

Figure 6.

Figure 6

Schematic representation of initial process of TPRT in the R1 element. (Top) First, R1-EN cleaves the non-coding bottom strand of 28S rDNA in the A–C junction. Boxes represent the 14 bp of TSD. (Middle) Then, the target DNA is partially denatured, allowing the UGU on the RNA template to base-pair with the loose target DNA. The template RNA is indicated by a gray line. In this model, the read-through 28S rRNA sequence is base-paired with the DNA target in longer region. During this process, RT of R1Bm may recognize the 3′UTR and base-paired region (open arrow), and place the RNA template at the accurate position for initiation of reverse transcription. (Bottom) Next, reverse transcription starts from the position next to the UGU sequence of template RNA, using the 3′-OH of A residue as primer.