Figure 4.

Kinetics and requirements for unfolding of the SEB isomers. Mixed complexes were assembled using the transposon ends illustrated below each panel. There was a 4-fold molar excess of the outside end present. The outside end was prepared by digesting pRC98 with AccI+ScaI (84 bp transposon arm/75 bp flanking DNA). The precleaved outside end was prepared by digesting pRC35 with BstEII+PvuII (85 bp transposon arm). The even-end DNA fragment was prepared by digesting pRC100 with AccI+BamHI (73 bp transposon arm/39 bp flanking DNA). The precleaved even-end was prepared by digesting pRC99 with AccI+PvuII (73 bp transposon arm). Other details are as given in Figure 2.