Figure 5.

The cleavage step is biased toward the αSEB isomer. The standard complex assembly reaction was scaled up and initiated at time zero by the addition of Mg⁺⁺. Aliquots were removed at the indicated times and loaded directly onto the gel that was under electrical tension. The outside end and even-end DNA fragments were prepared by digesting pRC167 and pRC100 with PstI+XhoI and HindIII+SpeI, respectively. To preclude any potential influence on the rate of cleavage by the sequence of bases flanking the transposon, the flanking DNA on each fragment is isogenic. The outside and even-ends are also isogenic out to bp 19 of the transposon end. Other details are as given in Figure 2.