Skip to main content
. 2023 Dec 6;11(12):1825. doi: 10.3390/vaccines11121825

Figure 4.

Figure 4

Monocyte, DC and neutrophil compartment frequency analysis. AAV-HBV-transduced C57BL/6 mice were treated with GalNAc-HBV siRNA and therapeutic vaccine, with GalNAc-control siRNA and therapeutic vaccine, and with GalNAc-control siRNA and mock vaccine. Intrahepatic immune cells from the liver were isolated 1 week after the administration of the second therapeutic vaccine (timepoint day 70) or 7 weeks after the fourth therapeutic vaccine (timepoint day 154). (A) Dotplot from scaled expressed hallmark genes for the antigen-presenting cell and monocyte compartments. (B) Frequency of macrophages across groups and statistical comparison at day 154 (paired Wilcox test, non-parametric, fdr adjustment). Macrophages at day 154 from GalNAc-HBV siRNA and therapeutic vaccine vs. mock were significant (p = 0.029, p.adj = 0.0435), and from GalNAc-HBV siRNA and therapeutic vaccine vs. GalNAc-control siRNA and therapeutic vaccine (p = 0.029, p.adj = 0.0435). Frequency of cDC1 across groups and statistical comparison at day 154 (paired Wilcox test, non-parametric, fdr adjustment). cDC1 at day 154 from GalNAc-HBV siRNA and therapeutic vaccine vs. GalNAc-control siRNA and therapeutic vaccine were significant (p = 0.029, p.adj = 0.087). (C) Frequency of neutrophils across groups and statistical comparison at day 154 (paired Wilcox test, non-parametric) with fdr adjustment showed no significant comparisons. Significant comparisons are highlighted with *. Boxplot outlier values are represented by a circle.