Table 1.
Apparent Kd values for the different substrate variants at different Ca2+ concentrations as indicated
| Substrate | appKd (μM) | ||
|---|---|---|---|
| 40 mM | 160 mM | 400 mM | |
| pATSerUGG/C | 0.11 ± 0.056 | 0.020 ± 0.0093 | 0.026 ± 0.018 |
| pATSerUGU/A | 1.3 ± 0.078 | 0.13 ± 0.023 | 0.030 ± 0.024 |
| pATSerUGU/DAP | 4.8 ± 1.2 | 0.73 ± 0.49 | 0.94 ± 0.39 |
| pATSerCGG/C | 11 ± 7 | 0.24 ± 0.06 | 0.057 ± 0.027 |
| pATSerCGU/A | n.d. | 3.2 ± 0.38 | 1.4 ± 0.8 |
| pATSerCGU/DAP | n.d. | 0.40 ± 0.077 | 0.21 ± 0.08 |
| pGlnU/A | 0.98 ± 0.18 | n.d. | n.d. |
| pGlnG/C | 0.015 ± 0.005 | n.d | n.d. |
| pSu3G/C | 0.079 ± 0.044 | n.d. | n.d. |
| pSu3U/A | 0.14 ± 0.043 | n.d. | n.d. |
The experiments were performed as described in Materials and Methods. Each value is an average of several independent experiments and n.d. = not determined. Underlined residues correspond to the identity of the +1/+72 base pair.