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Expression and processing of the products of various pilS mutants. Whole extracts of E. coli cells harboring pKK641A′ pilS1 and the mutant pilS plasmids were separated by SDS-PAGE and subjected to Western blot analysis using antipilin antiserum. Lanes: 1, pKK692 (wild-type pilS); 2, without pilS plasmid; 3, without pKK641A′ pilS1; 4 to 6, pKK693 to pKK695; 7 to 35, pKK692 with the indicated pilS mutations. The positions of prepilin (22 kDa) and pilin (19 kDa) are indicated on the right.
